Repeat the steps with additional samples.Fill a cuvette ¾ full with the filtered leaf extract.Filter the solution into a labeled test tube using the filter paper and funnel.Grind the mixture with the pestle for 2-3 minutes until the mixture is homogenized as much as possible.Place the sample in the clean mortar and add 20 mL of 95% ethanol.If possible, avoid veins and stems by cutting out small leaf tissue sections with scissors. Using a balance, measure 0.50 g of each leaf sample.Analyzing the absorption spectrum (what wavelengths are absorbed versus transmitted) can tell us what pigments are present in a leaf and what wavelengths of light will support the photosynthetic process. This happens when leaves stop producing chlorophyll, causing other pigments such as carotenoids or anthocyanins to become visible. As the seasons change, some leaves change from green to red, orange, yellow, and brown. This is similar to the energy absorption that occurs when wearing a dark T-shirt on a sunny day (which is why doing so will make you much warmer than wearing a light-colored shirt). What colors of light or wavelengths do chloroplasts actually need for this process? The leaves of many plants are green, which indicates that most of the green light falling on leaves is being reflected or transmitted, since you can see it! Other colors are absorbed by the leaf. Plants use light energy to synthesize energy-rich chemical compounds like sugars and starches from carbon dioxide (CO 2) and water (H 2O). Learn how to use the Analyze Solution page by performing an experiment on absorption. Analyzing the absorption spectrum of leaves
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